eeDAP Github website: https://github.com/DIDSR/eeDAP
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MicroRT Registration

A. Low Resolution Registration (More info on Low Resolution Page)
1. Place selected tissue glass (label_pos) onto the stage and change lens (mag_lres) according to the input file
2. Move stage using the joystick and center and focus a unique location (that stands out on the thumb WSI) on the tissue and click Take Stage Position
3. Click the same unique location on the thumb WSI and click Take WSI Position
4. Check results, Click on "Registration is Good!" if the registration is accurate
5. If results are accurate, repeat steps 2,3, and 4 with 2 other unique points
6. Select Load last calibration (Zoomed in image will appear)
* To skip High Resolution Registration and use only Low Resolution Registration, click on Finish Registration (Not recommended)
 
B. High Resolution Registration (More info on High Resolution Page)
1. Change lens (mag_hres) according to input file, the WSI will show the zoomed up area on the screen
2. Click on GoTo Position, the stage will move automatically to previously taken Stage Position (Low Resolution)
3. Adjust and center the stage using the joystick to a unique location in the same ROI of the low resolution registration (that stands out on the thumb WSI) and click Take Stage Position
4. Click the same unique location on the thumb WSI and click on Take WSI Position
5. Check results, Click on "Registration is Good!" if the registration is accurate
6. Repeat steps 2,3,4, and 5 with the 2 other unique points
 
 
*It this is the 1st time, do not skip. If done before, press Skip Offset
1. In the camera image, move the center of reticle to one small cell
2. Click Feature Centered in Camera
3. Look through the eyepiece, move the center of reticle to the same small cell
4. Click Feature Centered in Eyepiece
 
Made with help of Dr.Explain