eeDAP Github website: https://github.com/DIDSR/eeDAP
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eeDAP: Evaluation Environment for Digital and Analog Pathology,     Types of Microscopes

Types of Microscopes

There are currently only two microscopes that we have used with the software.

The basic microscope requirements are the following:
1) It has a trinocular tube: a camera can be mounted and the camera view is the same as the eyepiece view.
2) It is compatible with one of the computer controlled stages.
 

Note: Kohler illumination calibration tutorial

https://www.microscopyu.com/tutorials/kohler

 
Name and model
Description

Axioplan 2 Imaging microscope with an Axiophot 2 head

 
Specifications and Manaul
(https://github.com/DIDSR/eeDAP/blob/master/000_docs/hardware_manual/Microscope/Axioplan2_specification_and_manual.pdf)
  • Field Number (FN) of the oculars (eyepieces) = 23mm 
  • Magnification of eyepiece (mag_e) = 10x 
  • Magnification of objective (mag_o) = 2.5x, 5x, 10x, 20x, 40x
  • FOV at Mag_o = FNmm/mag_o 
  • FOV at 2.5x = 23mm/2.25 = 9.200mm
  • FOV at 5x = 23mm/5 = 4.600mm
  • FOV at 10x = 23mm/10 = 2.300mm
  • FOV at 20x = 23mm/20 = 1.150mm 
  • FOV at 40x = 23mm/40 = 0.575mm
  • Apparent diameter of microscope image "at 25cm" = FN*mag_e = 23cm
Diameter for ocular micrometer (reticle) = 26mm

Olympus BX43

 
Specifications and Manaul
(https://github.com/DIDSR/eeDAP/blob/master/000_docs/hardware_manual/Microscope/olympus_specification_and_manual.pdf)
  • Field Number (FN) of the oculars (eyepieces) = 22mm
  • Magnification of eyepiece (mag_e) = 10x 
  • Magnification of objective (mag_o) = 2x, 4x, 10x, 20x, 40x
  • FOV at Mag_o = FNmm/mag_o
  • FOV at 2x = 22mm/2 = 11.00mm 
  • FOV at 4x = 22mm/4 = 5.50mm
  • FOV at 10x = 22mm/10 = 2.20mm
  • FOV at 20x = 22mm/20 = 1.10mm
  • FOV at 40x = 22mm/40 = 0.55mm 
  • Apparent diameter of microscope image "at 25cm" = FN*mag_e = 22cm 
  • Diameter for ocular micrometer (reticle) = 24mm
 
Made with help of Dr.Explain

Unregistered version