eeDAP: Evaluation Environment for Digital and Analog Pathology, Alignment
Alignment
Depending on hardware, you may have the ability to completely align the camera with the eyepiece by rotating and shifting the camera. This process is made possible by loosening and tightening one - three screws on the camera mount. This can only be done while setting up the equipment and before beginning the experiment. The alignment should persist over time and need to none little adjustments unless the screws are ineffective or the system is bumped. A calibration slide, reticle, and virtual reticle are very useful for this alignment. A good camera mount also has a focus screw that can be adjusted so that the camera and the eyepiece are nearly in focus at the same time.
1. Place the grid slide on the stage and change to the highest magnification optics (Figure 1)
2. Look through the eyepiece and focus the microscope.
3. Still looking through the eypiece move stage to let the reticle just touch the boundary of the gird corner.
4. Depending on your camera:
a. If you are able focus your camera, don't focus camera image using your microscope
b. If you are unable to focus your camera, focus camera image using your microscope
5. Loosen the screws around camera. (Figure 3)
6. Use your hand to adjust the camera to make the reticle in camera image just touch the same boundary of the gird corner.(Figure 2)
7. Hold the camera and while looking at the camera image, start tighting the screws until it is aligned.
*Note: 1. Small shift could be solved by eyepiece and camera register processing.
2. Large shift might influenct the fast registration during task. Because our fast registration use a small center area of camera image, lager shift between eyepiece and camera will lead outstanding feature goes out of the camera registration area. Best registration is another method to slove the large shift problem.
